405 nm Notch Optical Filter For Flow Cytometry

A 405 nm notch optical filter is an optical filter designed to block or attenuate light at a specific wavelength of 405nm. This is achieved through the use of a specialized coating or material that is applied to the surface of the filter. This type of filter is commonly used in various applications such as fluorescence microscopy, flow cytometry, and laser-based spectroscopy.

One of the key advantages of our 405nm notch filter is its ability to improve contrast and reduce background noise in fluorescence imaging applications. By blocking unwanted excitation light at 405nm, our filter can effectively enhance the signal-to-noise ratio of fluorescence images and improve the overall quality of the data. However, it can also work perfectly in other applications. Let us know your intended needs; our experts will offer you the best optics solutions!

Features

- Notch filter for NF405nm

- High attenuation

- Low insertion loss

- Adjustable wavelength

- Stable environmental coating

- ISO9001, RoHS certified

Parameters

Spectrum transmission curve

Applications

With its ability to selectively block light at a specific wavelength, this filter can help to improve the quality and reliability of a wide range of optical measurements and imaging applications. Here are some examples:

- Fluorescence microscopy

- Flow cytometry

- Laser-based spectroscopy applications

- optical coherence tomography

How does a 405 nm notch optical filter apply in flow cytometry?

Flow cytometry is an essential technique used in cell biology to analyze and sort cells. It involves using laser light to excite fluorophores in a sample, emitting light at different wavelengths that the instrument can detect. However, a common problem in flow cytometry is the presence of unwanted signals, such as laser scatter and autofluorescence, which can interfere with accurately detecting the motion of interest.

To overcome this issue, optical filters are used to selectively block unwanted wavelengths of light while allowing the signal of interest to pass through. One of the most commonly used filters in flow cytometry is the 405nm optical notch filter.

The 405nm optical notch filter is designed to block the 405nm laser scatter and autofluorescence, which are commonly observed in flow cytometry experiments. The filter consists of a series of dielectric layers that are deposited on a glass or quartz substrate. These layers are designed to reflect the 405nm wavelength while allowing other wavelengths to pass through.

When the laser light passes through the 405nm optical notch filter, the unwanted 405nm signals are reflected, while the signal of interest is transmitted through the filter to the detector. This results in a cleaner signal with reduced noise, which improves the accuracy and sensitivity of the flow cytometry experiment.

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