273nm 275nm UV Bandpass Filter Glass For Fluorescence Microscope

273nm 275nm UV Bandpass Filter Glass are designed to allow only ultraviolet light with CWL 273nm and 275nm to pass through. These filters are commonly used in applications such as fluorescence microscopy, DNA analysis, and disinfection.

 

Our 275nm UV Bandpass Filter Glass features a highly accurate central wavelength of 275nm and a customizable full width at half maximum (FWHM) of 10nm with an optical density (OD) of 2-6. They are often made from quartz or specialized materials designed to block out unwanted wavelengths. Our team can work with you to create bespoke optical systems optimized for specific applications.

 

Features

- Narrow/Broad bandpass for 275nm

- Accurate wavelength

- Highly scratch-resistant

- High signal-to-noise ratio

- Deeply customized

- High transmittance

- ISO9001 certified

 

Parameters

Product Name	275nm UV bandpass filter
Central Wavelength (CWL)	275nm
FWHM	10nm or custom
Diameter	20mm or custom
Thickness	1mm or custom
Transmission Rate	>90%
Blocking depth	OD3 or custom
Coating	Hard-film coating
Tolerance	±0.01-0.1mm
Wavelength Range:	200-1100nm

 

Spectrum transmission curve

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Applications:

275nm UV bandpass filter has tremendous applications for controlling and manipulating light in scientific, industrial, and medical fields. Some of the common uses are:

- Fluorescence microscopy

- Photolithography

- Sterilization in medical facilities

- Water purification

- Environmental monitoring

- Scientific research in protein analysis, DNA analysis, and disinfection.

 

How does 275nm UV Bandpass Filter apply in Fluorescence Microscope?

In fluorescence microscopy, a 275nm UV bandpass filter is commonly used as an excitation filter. This filter allows only UV light with 275nm wavelengths to pass through and excite fluorescent molecules in a sample. The excited molecules then emit light at longer wavelengths, which can be captured and visualized using appropriate emission filters and a detector such as a camera.

 

Fluorescence microscopy is a widely used technique in the biological and biomedical sciences for visualizing structures and processes within cells and tissues. It allows researchers to selectively label specific molecules or structures with fluorescent probes, which can be imaged using appropriate filters and detectors.

 

The 275nm UV band pass filter is beneficial because it can excite specific fluorescent probes that are only excited by UV light. For example, DAPI (4',6-diamidino-2-phenylindole) is a commonly used fluorescent dye excited by UV light with a peak wavelength of around 358nm. However, the excitation light at this wavelength can also cause background fluorescence from cellular components such as mitochondria and lysosomes. Using a 273nm 275nm UV Bandpass Filter Glass, which is closer to the optimal excitation wavelength of DAPI, but with a narrower wavelength range, researchers can reduce background fluorescence and improve the signal-to-noise ratio.

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