Blue Bandpass Filter 415nm Optical Filter For Flow Cytometry

Blue bandpass filter 415nm is an optical filter that only allows light in the blue part of the spectrum to pass through. This filter is often used in scientific and industrial applications where precise control of the wavelength of light is necessary, particularly useful for applications involving fluorescence, such as in microscopy or flow cytometry.

 

Our filter has a high transmission of over 90%, with high optical density outside of the passband to block out unwanted wavelengths effectively. Moreover, the filters are made from high-quality materials and durable coatings that are virtually resistant to damage from handling, cleaning, and exposure to environmental factors such as temperature and humidity. For more information, please send us an inquiry.

 

Features

- Bandpass for 415nm

- Accurate wavelength

- Highly scratch-resistant

- High signal-to-noise ratio

- Deeply customized

- High transmittance

- ISO9001, ROHS certified

 

Parameters

Product Name	415nm bandpass filter
Central Wavelength (CWL)	415nm
FWHM	30nm or custom
Diameter	25mm or custom
Thickness	1mm or custom
Transmission Rate	>90%
Blocking Depth	OD6 or custom
Coating	Hard-film coating
Tolerance	±0.01-0.1mm
Wavelength Range:	200-1100nm

 

Spectrum transmission curve

 

Applications

Blue bandpass filters 415nm have applications in scientific and industrial settings, particularly in fields such as microscopy, spectrophotometry, and fluorescence imaging. Here are some typical applications:

- Fluorescence microscopy

- Flow cytometry

- Spectrophotometry

- Lighting applications

 

How does the blue bandpass filter 415nm apply in flow cytometry?

Flow cytometry is a technique used to analyze and sort cells based on their physical and chemical properties. Fluorescence-based flow cytometry uses fluorescent dyes or proteins to label specific cell components, allowing for their detection and analysis. We can use blue bandpass filter 415nm in flow cytometry in several ways:

 

Excitation: Many fluorescent dyes used in flow cytometry are excited by blue light. A blue laser with a wavelength of 488nm is commonly used to excite these dyes, and a 415nm bandpass filter can select only the blue fluorescence for detection.

 

Detection: After excitation, the fluorescent signal emitted by the labeled cells is collected and detected by the flow cytometer. Blue bandpass filters can selectively detect the blue fluorescence emitted by the labeled cells while blocking out other unwanted wavelengths.

 

Compensation: In flow cytometry, the spectral overlap between fluorescent dyes can lead to false-positive or false-negative results. Compensation is used to correct this overlap. Blue bandpass filters can be used to separate the signals from different shades excited by the same laser by using filters with different wavelengths and bandwidths.

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